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Environmental analysis method to guide aquaculture sustainable development: case study in Mayotte marine natural park, Killian Chary [et al.]

Environmental analysis method to guide aquaculture sustainable development: case study in Mayotte marine natural park, Killian Chary [et al.]

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that can be applied to other species or territories and that can be useful for decision makers to

guide aquaculture development.



181



Nutritional evaluation of two types of

euryhaline rotifers Brachionus plicatilis sp.

complex and digestive enzyme response at

first feeding in Japanese Flounder,

Paralichthys olivaceus

Viliame Waqalevu



∗† 1



, Shojiro Miyauchi 2 , Shuo Zhang 2 , Tomonari

Kotani 3



1



United Graduate School of Agricultural Sciences, Kagoshima University – United Graduate School of

Agricultural Sciences, Kagoshima University Korimoto 1-21-24, Kagoshima City, Kagoshima Prefecture,

890-0065, Japan

2

Graduate School of Fisheries, Kagoshima University – Faculty of Fisheries, Kagoshima University

Shimoarata 4-50-20, Kagoshima City, Kagoshima Prefecture, 890-0056, Japan

3

Faculty of Fisheries, Kagoshima University (http://www.fish.kagoshima-u.ac.jp/efish/) – Faculty of

Fisheries, Kagoshima University Shimoarata 4-50-20, Kagoshima City, Kagoshima Prefecture, 890-0056,

Japan



It is well established that aquaculture can provide solutions in relieving pressure applied to

wild stocks due to heavy consumer demand that often leads to a cascade of ecological effects.

The bottleneck in hatcheries in many developing countries lies in reliable production of live

feed suitable for the important stage of first exogenous feeding. Rotifers occur naturally in the

wild and due to many of its suitable characteristics, it is a preferred source of nutrition during

first feeding of finfish larvae. In this study, B. plicatilis (Obama strain) and B. rotundiformis

(Indonesian strain) were enriched with microalgae Chlorella vulgaris and Salmon roe derived

oil emulsion and fed to Japanese flounder until 7 days after hatching (DAH). Analysis were

conducted to assess the viability of these enrichment media in terms of its (i) essential fatty

acids content such as arachidonic acid (C20:4 n-6; ARA), eicosapentaenoic acid (C20:5 n-3;

EPA) and docohexaenoic acid (C22:6 n-3; DHA), vital for the development of early stage fish

larvae and (ii) differences in the induction of digestive protein breakdown in first feeding stages

of the Japanese flounder. It was found that DHA enriched C. vulgaris and salmon roe emulsion

oil contain high fatty acid composition of essential fatty acids that were absorbed by both rotifer

species. It was also found the smaller sized B. rotundiformis induced higher protein hydrolysis

in 5 DAH larvae and this activity switched to L-type at 7 DAH. This may be reflective of

the preference of the larvae and/or metabolic changes linked to metamorphosis that it would

soon undergo in its life cycle. There are hopes that parallels from this study on nutritional

requirements and digestive enzymatic activity can be used to develop knowledge on the culture

of the economically important and difficult to culture, Leopard Coral Trout, Plectropomus

leopardus, at the moment of first exogenous feeding.







Speaker

Corresponding author: vwaqalevu@hotmail.com



182



Treatment of bacterial disease in fish

aquaculture by natural products from

French Polynesia

Tepoerau Mai ∗ 1 , Raimana Ho 2 , Ali Al-Mourabit 3 , Phila

Raharivelomanana 1 , Denis Saulnier 4 , Cecile Debitus 5

1



3



Universit´e de la Polyn´esie fran¸caise (UPF) – BP 6570, 98 702 FAA’A, Tahiti, French Polynesia

2

Universit´e de la Polyn´esie fran¸caise – BP 6570, 98 702 FAA’A, Tahiti, French Polynesia

Centre National de Recherche Scientifique (CNRS) – CNRS – Gif sur Yvette, Avenue de la Terrasse,

France

4

IFREMER – BP 6570, 98 702 FAA’A, Tahiti, French Polynesia

5

LEMAR – Laboratory IUEM LEMAR Technopole Brest – Technopˆole Brest-Iroise, rue Dumont

d’Urville, 29280 Plouzan´e, France



In French Polynesia, fish aquaculture of Platax orbicularis is a sector in emergence. However

bacterial infection by Vibrio harveyi and Tenacibaculum maritimum limits its development. This

study aims to identify if natural products from French Polynesia can be an efficient biosourcing

to prevent bacterial infection in the aquaculture of P. orbicularis. Methodology consisted in a

screening of live organisms in the inhibition of V. harveyi quorum sensing, then the isolation

of natural products from these organisms and the identification of quorum sensing inhibitors.

Two natural products sourcing were studied, the plant Morinda citrifolia and the marine sponge

Leucetta chagosensis. Derivatives of aminoimidazole from Leucetta chagosensis were identified

as quorum sensing inhibitors and lyophilized powder of M. citrifolia was shown to protect fishes

from bacteria in vivo. These results could be used as an alternative of antibiotic supply to

prevent bacterial diseases in fish aquaculture.







Speaker



183



C1/ The role of fishes on coral reefs



184



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